Full transcriptome analysis of Chinese Hamster Ovary cell lines producing a dynamic range of Coagulation Factor VIII
نویسندگان
چکیده
Background and novelty Coagulation Factor VIII (FVIII) is an essential cofactor in the blood coagulation cascade. Inability to produce functional FVIII results in haemophilia A which can be treated with recombinant FVIII [1]. Chinese Hamster Ovary (CHO) cells are the most used cell line for producing complex biopharmaceuticals due to its ability to perform complex post-translational modifications. When mammalian cells overexpress a protein like FVIII they will adapt by regulating various proteins and pathways to support synthesis/production of this protein. Yields of FVIII produced in CHO are low and for this reason a greater understanding of what constitute a high producing cell line is desired. In this study a full transcriptome analysis was undertaken in order to analyze the differences between high and low producers of FVIII
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